منابع مشابه
LETM1 in mitochondrial cation transport
LETM1 (leucine zipperEF handcontaining transmembrane 1) encodes a highly conserved eukaryotic protein of the mitochondrial inner membrane which is essential to control mitochondrial volume homeostasis. Indeed, LETM1 RNA interference causes severe mitochondrial changes that include massive matrix swelling, loss of cristae structure and network fragmentation in S. cerevisiae (Nowikovsky et al., 2...
متن کاملLETM1-Mediated K+ and Na+ Homeostasis Regulates Mitochondrial Ca2+ Efflux
Ca2+ transport across the inner membrane of mitochondria (IMM) is of major importance for their functions in bioenergetics, cell death and signaling. It is therefore tightly regulated. It has been recently proposed that LETM1—an IMM protein with a crucial role in mitochondrial K+/H+ exchange and volume homeostasis—also acts as a Ca2+/H+ exchanger. Here we show for the first time that lowering L...
متن کاملFunctional reconstitution of the mitochondrial Ca2+/H+ antiporter Letm1
The leucine zipper, EF hand-containing transmembrane protein 1 (Letm1) gene encodes a mitochondrial inner membrane protein, whose depletion severely perturbs mitochondrial Ca(2+) and K(+) homeostasis. Here we expressed, purified, and reconstituted human Letm1 protein in liposomes. Using Ca(2+) fluorophore and (45)Ca(2+)-based assays, we demonstrate directly that Letm1 is a Ca(2+) transporter, w...
متن کاملCation Transport in Yeast
1. The distribution of azide added to suspensions of bakers' yeast was studied under various conditions. The recovery of azide was estimated in the volume of water into which low concentrations of electrolytes can readily diffuse (anion space). Considerable azide disappeared from this anion space. 2. The incomplete recovery of azide in the anion space is due to its uptake by the cells. This upt...
متن کاملEvidences for a new cation channel in the brain mitochondrial inner membrane
Introduction: Previous studies and our works have indicated several cation channels in the rat brain mitochondrial inner membrane. In this work, we report the single-channel characterization of a cation channel from the rat brain mitochondrial inner membrane incorporated into a planar lipid bilayer. Methods: After removing and homogenizing the adult rat brain, its supernatant was centrifuged...
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ژورنال
عنوان ژورنال: Frontiers in Physiology
سال: 2014
ISSN: 1664-042X
DOI: 10.3389/fphys.2014.00083